Production of Reducing Sugars from Oil Palm Stems through Acid Hydrolysis Process with Variations in Hydrochloric Acid (HCl) Concentrations
DOI:
https://doi.org/10.32493/jitk.v10i2.60980Keywords:
oil palm trunk, acid hydrolysis, hydrochloric acid, reducing sugar, second-generation bioethanolAbstract
Oil palm trunks are one of the most abundant types of plantation waste and contain cellulose and hemicellulose that can be converted into reducing sugars through an acid hydrolysis process. This study aimed to evaluate the effect of hydrochloric acid (HCl) concentration on reducing sugar production from oil palm trunk hydrolysate. The experiment employed three HCl concentration treatments, namely 1 M, 2 M, and 3 M, each with three replications. Before hydrolysis, the samples were pretreated using 1 M NaOH solution and subsequently hydrolyzed at 80–90°C for 100 minutes. The parameters observed included total dissolved solids (Brix), measured using a refractometer, and reducing sugar content, determined using the Nelson-Somogyi method. The results showed that increasing HCl concentration enhanced sugar production. The average Brix value increased from 6% at 1 M HCl to 13% at 3 M HCl, while the reducing sugar content increased from 366.25 ppm to 508.07 ppm. Therefore, oil palm trunk has considerable potential as a feedstock for bioethanol production, and the 3 M HCl treatment was found to be the most effective hydrolysis condition in this study.
References
Sensors and Instruments for Brix Measurement: A Review. Sensors, 22(6), 1–20. https://doi.org/10.3390/s22062290
Li, N., Wang, J., Wang, B., Huang, S., Hu, J., Yang, T., Asmutola, P., Lan, H., & Qinghui, Y. (2021). Identification of the Carbohydrate and Organic Acid Metabolism Genes Responsible for Brix in Tomato Fruit by Transcriptome and Metabolome Analysis. Frontiers in Genetics, 12, 714942. https://doi.org/10.3389/fgene.2021.714942
Ma’mun, S., Rochmaningsih, N. A., & Nabila, R. (2024). Identifikasi Gugus Gula Pereduksi Mikroalga Arthrospira platensis Hasil Hidrolisis dengan Bantuan Gelombang Mikro Identification of Reducing Sugars in the Microalga Arthrospira platensis from Microwave-assisted Hydrolysis. JT; Jurnal Teknik, 13(01), 30–37. http://jurnal.umt.ac.id/index.php/jt/index
Mawarda Rilek, N., Hidayat, N., & Sugiarto, Y. (2017). Hidrolisis Lignoselulosa Hasil Pretreatment Pelepah Sawit (Elaeis guineensis Jacq) menggunakan H2SO4 pada Produksi Bioetanol. Industria: Jurnal Teknologi Dan Manajemen Agroindustri, 6(2), 76–82. https://doi.org/10.21776/ub.industria.2017.006.02.3
Nasiri, J., Kiani, F., & Koohyar, F. (2018). Refractometric study on binary, ternary, and quaternary solutions made by water, methanol, ethanol, glycerol, D-glucose monohydrate (DGMH), sucrose, and sodium chloride at T = 293.15 K and atmospheric pressure. Journal of the Mexican Chemical Society, 62(4), 160–176. https://doi.org/10.29356/jmcs.v62i4.679
Ocreto, J. B., Chen, W. H., Ubando, A. T., Park, Y. K., Sharma, A. K., Ashokkumar, V., Ok, Y. S., Kwon, E. E., Rollon, A. P., & De Luna, M. D. G. (2021). A critical review on second- and third-generation bioethanol production using microwaved-assisted heating (MAH) pretreatment. Renewable and Sustainable Energy Reviews, 152, 111679. https://doi.org/10.1016/J.RSER.2021.111679
Purnawan, A., Thontowi, A., Kholida, L. N., & Perwitasari, U. (2021). Review Hidrolisis Biomasa Lignoselulosa Untuk Xilitol. Jurnal Ilmu Lingkungan, 19(3), 485–496. https://doi.org/10.14710/jil.19.3.485-496
Rysha, A., & Mulaj, L. (2026). Quantitative divergence between refractometric total soluble solids (°Brix) and label-declared values in commercial fruit beverages: implications for screening. Scifood, 20, 351–360. https://doi.org/10.5219/scifood.105
Santanaraj, J., Sajab, M. S., Mohammad, A. W., Harun, S., Chia, C. H., Zakari, S., & Kaco, H. (2017). Enhanced delignification of oil palm empty fruit bunch fibers with in situ fenton-oxidation. BioResources, 12(3), 5223–5235. https://doi.org/10.15376/biores.12.3.5223-5235
Świątek, K., Gaag, S., Klier, A., Kruse, A., Sauer, J., & Steinbach, D. (2020). Acid hydrolysis of lignocellulosic biomass: Sugars and furfurals formation. Catalysts, 10(4), 1–18. https://doi.org/10.3390/catal10040437
Tareen, A. K., Punsuvon, V., & Parakulsuksatid, P. (2020). Investigation of alkaline hydrogen peroxide pretreatment to enhance enzymatic hydrolysis and phenolic compounds of oil palm trunk. 3 Biotech, 10(4), 179. https://doi.org/10.1007/s13205-020-02169-6
Thahir, M. T., Rina, & Husna, S. (2024). Pembuatan dan Karakterisasi Bioetanol dari Limbah Jerami Melalui Proses Hidrolisis dan Fermentasi. Jurnal Sains Dan Teknik Terapan, 2(1), 9–19. https://journal.akom-bantaeng.ac.id/index.php/jstt
Zhou, Z., Liu, D., & Zhao, X. (2021a). Conversion of lignocellulose to biofuels and chemicals via sugar platform: An updated review on chemistry and mechanisms of acid hydrolysis of lignocellulose. Renewable and Sustainable Energy Reviews, 146, 111169. https://doi.org/10.1016/J.RSER.2021.111169
Zhou, Z., Liu, D., & Zhao, X. (2021b). Conversion of lignocellulose to biofuels and chemicals via sugar platform: An updated review on chemistry and mechanisms of acid hydrolysis of lignocellulose. Renewable and Sustainable Energy Reviews, 146, 111169. https://doi.org/10.1016/J.RSER.2021.111169
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