VOLTAMMETRIC DETERMINATION OF GENOMIC DNA USING GOLD-THIOL SELF-ASSEMBLED MONOLAYER ELECTRODE

Abstract

Electrochemical methods have sensitivity, miniaturization capability, low cost and make them suitable for development of portable detection devices. The voltammetric determination of M. tuberculosis dsDNA was carried out in this study using gold electrode modified by SAM of thiols. The DPV responses of guanine of dsDNA was found at 0.2 V. The voltammogram showed a linear correlation between the current peak 2 of guanine and the concentration of dsDNA over the concentration range 1–25 ppm (Y = 1.108X - 0.851, R =0,993). This electrochemical method desire gives slightly different results with biophotometri.

Keywords: M.tuberculosis, gold electrode, guanine oxidation, self-assembled monolayer, voltammetry